Defining the diverse spectrum of inversions, complex structural variation,...
March 2017 Authors: Ryan Collins, Harrison Brand, Claire Redin, Carrie Hanscom, et al. Info: Scientists at Harvard and other institutions sequenced nearly 700 individuals with autism spectrum disorder...
View ArticleThe RNA Exosome Syncs IAV-RNAPII Transcription to Promote Viral Ribogenesis...
April 2017 Authors: Alexander Rialdi, Judd Hultquist, David Jimenez-Morales, Zuleyma Peralta, et al. Info: Scientists in New York and California teamed up to study the role of nuclear RNA exosomes in...
View ArticleTripartite species interaction: eukaryotic hosts suffer more from phage...
April 2017 Authors: Carolin Wendling, Agnes Piecyk, Dominik Refardt, Cynthia Chibani, et al. Info: Scientists investigated the extent to which a bacterium’s susceptibility or resistance to prophages...
View ArticleComparative Analysis of Ralstonia solanacearum Methylomes
April 2017 Authors: Ivan Erill, Marina Puigvert, Ludovic Legrand, Rodrigo Guarischi-Sousa, et al. Info: Scientists report results of analyzing epigenetic patterns in two strains of a plant pathogen....
View ArticleLong-Read Isoform Sequencing Reveals a Hidden Complexity of the...
June 2017 Authors: Dóra Tombácz, Zsolt Csabai, Attila Szűcs, Zsolt Balázs, et al. Info: Scientists from Hungary and California conducted isoform sequencing with PacBio to analyze the transcriptome of...
View ArticleClik here to view.
Creighton Lab Boosts Yield, Sequencing Efficiency with BluePippin
At Creighton University in Omaha, Neb., Dr. Anna Selmecki’s lab explores various fungal species to understand genome instability, pathogenesis, and the acquisition of drug resistance. For these...
View ArticleCondensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and...
June 2017 Authors: Kobe Yuen, Brian Slaughter, and Jennifer Gerton Info: Scientists in Kansas used the 4C-seq method to study the chromosome complex condensin II in mouse and human genomes. They found...
View ArticleThe Conserved RNA Binding Cyclophilin, Rct1, Regulates Small RNA Biogenesis...
June 2017 Authors: An-Yun Chang, Stephane Castel, Evan Ernst, Hyun Soo Kim, Robert Martienssen Info: Cold Spring Harbor Laboratory researchers studied the Rct1 protein in S. pombe and found that it is...
View ArticlePacBio Users: Size Selection Is Essential for Generating Excellent Results
The Sage Science team was delighted to attend and co-sponsor PacBio’s annual East Coast user group meeting in Baltimore last week, particularly since there was a half-day session devoted to our...
View ArticleDual redundant sequencing strategy: Full-length gene characterisation of 1056...
August 2017 Authors: Albrecht V, Zweiniger C, Surendranath V, Lang K, et al. Info: Scientists in Germany describe a high-throughput approach for characterizing novel HLA alleles. The method involves...
View ArticleClik here to view.
What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass...
Customers may have noticed two very similar cassette definitions; 0.75%DF Marker S1 high-pass 4-10kb vs2 and 0.75%DF Marker S1 High-Pass 6-10kb vs3. Since these two protocols cover a similar range,...
View ArticleError rates, PCR recombination, and sampling depth in HIV-1 whole genome deep...
July 2017 Authors: Fabio Zanini, Johanna Brodin, Jan Albert, Richard Neher Info: Researchers in Germany and Sweden expounded on a previous deep sequencing study of HIV-1 populations to evaluate...
View ArticleTranscriptome Profiling Using Single-Molecule Direct RNA Sequencing Approach...
July 2017 Authors: Qingshan Xu, Junyan Zhu, Shiqi Zhao, Yan Hou, et al. Info: Scientists from Anhui Agricultural University in China performed long-read transcriptome sequencing of the tea plant...
View ArticleComplete Genome Sequences of Seven Strains Composing a Model Bacterial...
September 2017 Authors: Ben Niu and Roberto Kolter Info: Scientists from Harvard Medical School released the full genome sequences of seven bacterial species they chose for a model community to...
View ArticleComprehensive Transcriptome Profiling of Balding and Non-Balding Scalps in...
October 2017 Authors: Yun-Ji Kim, Byulee Yoon, Kyudong Han, Byung Cheol Park Info: Scientists in Korea compared transcriptomes of RNA extracted from balding and non-balding scalp regions on the same...
View ArticleAssessing a single targeted next generation sequencing for human leukocyte...
October 2017 Authors: Manish Gandhi, Deborah Ferriola, Curt Lind, Jamie Duke, et al. Info: Scientists from the Mayo Clinic, Children’s Hospital of Philadelphia, and other institutions teamed up to...
View ArticleLong-read sequencing of the coffee bean transcriptome reveals the diversity...
November 2017 Authors: Bing Cheng, Agnelo Furtado, Robert Henry Info: In this study, researchers generated the transcriptome of the tetraploid Arabica coffee bean, focusing on caffeine and sucrose...
View ArticleDe Novo Assembly of a New Solanum pennellii Accession Using Nanopore Sequencing
October 2017 Authors: Maximilian Schmidt, Alxander Vogel, Alisandra Denton, Benjamin Istace, et al. Info: Scientists used Oxford Nanopore technology to sequence Solanum pennellii, a wild species of...
View ArticlePreprint Roundup: Long-Read Sequencing, ddRAD-seq, and a New Look at Viruses
We always keep our eyes peeled for interesting new research from scientists using Sage Science automated DNA size selection instruments, and three recent preprints caught our attention. Here’s a look:...
View ArticleA Look Back at 2017
As we start to make lists of New Year’s resolutions (with bets on how long they’ll last), it’s the perfect time for a moment to absorb the themes and highlights of 2017. In our corner of the world,...
View Article